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Anonymous
Anonymous asked in Science & MathematicsBiology · 2 months ago

Biology calculations help?

You would like to make a solution with a final volume of 100 ul and only 10 ng/ul of DNA. In order to make this solution, you obtain a microcentrifuge tube with 89 ng/ul of DNA in buffer and a container of molecular biology grade water, that has no DNA. How many ul of the DNA/elution buffer solution and how many ul of the molecular biology grade water are you going to need to combine to make 100 ul of the 10ng/ul solution?

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  • 2 months ago
    Favorite Answer

    For the DNA:

    C1V1 = C2V2

    89 ng/uL X V1 = 10 ng/uL X 100 uL

    V1 = 11.2 uL

    So, you will use 11.2 uL of the DNA stock solution and 88.8 uL of water.

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