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Biology calculations help?
You would like to make a solution with a final volume of 100 ul and only 10 ng/ul of DNA. In order to make this solution, you obtain a microcentrifuge tube with 89 ng/ul of DNA in buffer and a container of molecular biology grade water, that has no DNA. How many ul of the DNA/elution buffer solution and how many ul of the molecular biology grade water are you going to need to combine to make 100 ul of the 10ng/ul solution?
- hcbiochemLv 72 months agoFavorite Answer
For the DNA:
C1V1 = C2V2
89 ng/uL X V1 = 10 ng/uL X 100 uL
V1 = 11.2 uL
So, you will use 11.2 uL of the DNA stock solution and 88.8 uL of water.