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What is the gas / bubbles coming out of the solution, when a flask with a broth solution containing ampicillin  is aerated with a stream of air through the flask? 

Why is this bubbling occurring in the solution? 

Update:

Maybe I should word it in a different way !! 

The bacteria broth solution had E. coli bacteria with added ampicillin 

When it was aerated it produced bubbles.

Why did it produce the bubbles???? I wondered if this was CO2 

Update 2:

Thank you Ted!!!!!!!

Answer 1

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What  do you mean by "aerating?"  Swirling the flask around while directing a stream of air over the top of the liquid?  Actually blowing air into the liquid?  If it's the  former, then the bubbles were CO2 being generated by the bugs as they consume the oxygen.  If the latter, then it's the same as what happens when you stick a straw in your drink and blow air through it.

Answer 2

Uh, you're aerating it with a stream of air. 

So, "air" and "it's being aerated"