How many (if any) tyrosines are there in this protein? You will need to use Beer's Law to solve this problem.?
A previously unknown protein has been isolated in your laboratory. Others in your lab have determined that the protein sequence contains 172 amino acids. They have also determined that this protein has no tryptophan and no phenylalanine.You have been asked to determine the possible tyrosine content of this protein. You know from your study of this chapter that there is a relatively easy way to do this. You prepare a pure 29.0 μM solution of the protein, and you place it in a sample cell with a 1 cm path length, and you measure the absorbance of this sample at 280 nm in a UV-visible spectrophotometer. The absorbance of the solution is 0.644.
How many (if any) tyrosines are there in this protein? You will need to use Beer's Law to solve this problem. The molar absorption coefficient of tyrosine is 1490 M-1cm-1.
Number of tyrosines in this protein =
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