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What size do you expect your expressed protein to be if the average amino acid is 110 Daltons?

Gene of Interest: malE sequence (Maltose Binding Protein, 50 nt/complete row)

1 Answer

  • Ted K
    Lv 7
    2 months ago
    Favorite Answer

    You didn't provide enough info.  All you give is "50 nt/complete row." How many rows????

    Assuming your sequence is the mature mRNA,

    1) Count up the total nucleotides [50/row * # rows] + [# nt's in the incomplete row]

    2) Divide by 3--that'll give the number of aa's

    3) Multiply #2 by 110. That'll give you your molecular size.

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