Chemistry molar concentration question?
SSPE (Saline/Sodium Phosphate/EDTA) is a commonly used hybridization buffer. It is used at 1X but the stock solution is usually made at 20X. The components of this buffer at 1X are 105.0 mM NaCl, 20.0 mM sodium phosphate (NaH2PO4) and 1.4 mM ethylenediametetraacetic acid (EDTA, disodium salt).
MW (NaCl) = 58.44 g/mol
MW (NaH2PO4) = 119.98 g/mol
MW (EDTA, disodium salt) = 372.24 g/mol
What is the molar concentration of NaCl at 20X?
What is the molar concentration of NaH2PO4 at 20X?
What is the molar concentration of EDTA, disodium salt at 20X?
- hcbiochemLv 73 weeks agoFavorite Answer
Just multiply the 1X concentrations by 20 and convert from mM to M by dividing by 1000.
NaCl: 105.0 mM X 20 / 1000 = 2.100 M
Repeat for the other 2 components.