chemistry question antibody dilution?

You've been given a solution containing 4.00 mg/mL mg/mL of anti-actin antibody. You need to incubate this antibody with 40.00 mL of actin solution at a 1:2000 dilution


How many µL of antibody would you add?

2 Answers

  • 4 weeks ago
    Favorite Answer

    4.00 mg/mL / 2000 = 2X10^-3 mg/mL

    C1V1 = C2V2

    4 mg/mL V1 = 2X10^-3 mg/mL (40 mL)

    V1 = 0.020 mL = 20 uL

    So, if you add 20 uL of the antibody stock solution to the 40.00 mL of actin solution, the antibody will be diluted 2000 times.

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  • Anonymous
    4 weeks ago

    What is your question?

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