chemistry question antibody dilution?
You've been given a solution containing 4.00 mg/mL mg/mL of anti-actin antibody. You need to incubate this antibody with 40.00 mL of actin solution at a 1:2000 dilution
How many µL of antibody would you add?
- hcbiochemLv 74 weeks agoFavorite Answer
4.00 mg/mL / 2000 = 2X10^-3 mg/mL
C1V1 = C2V2
4 mg/mL V1 = 2X10^-3 mg/mL (40 mL)
V1 = 0.020 mL = 20 uL
So, if you add 20 uL of the antibody stock solution to the 40.00 mL of actin solution, the antibody will be diluted 2000 times.
- Anonymous4 weeks ago
What is your question?