N asked in Science & MathematicsBiology · 1 month ago

Help with this biology question!!?


You quantify nDNA from a plant and get 115ng/ul from your nanodrop result. Assuming you want to add 2000 copies of a chloroplast DNA (cpDNA) locus to a PCR reaction, to what concentration should you dilute the DNA? Assume the genome size is 5 x 1011bp, ploidy is diploid, the chloroplast copy number ratio to nDNA is 50 to 1, and you’re adding 2uL of diluted DNA. H

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