You are a clinical immunologist and you are studying B-cells.?


Your mouse strains all visually look the same but they each have a homozygous deletion for a different gene.  

You need to identify which gene(s) is(are) knocked out in each mouse so you can label the cages appropriately. You don’t have time to wait for the sequencing results so you decide to look at the B-cells and antibodies instead.

Update 2:

For each mouse you measure whether the following are present: antibodies (of any type), IgG antibodies, pro B-cells, immature B-cells, and mature B-cells. Note: for this question, the amount does not matter, if a homozygous deletion would prevent the formation then they are NOT present, if a homozygous deletion of the gene would not completely prevent formation then they are present. 

What do you expect to see in your mice with the given deleted gene(s)? 

Update 3:


CD40 Ligand

Igα and Igβ (both are knocked out)



Basically, in a mouse with each of the gene(s) deletion above do you expect to see antibodies (of any type), IgG antibodies, pro B-cells, immature B-cells, and/or mature B-cells. Remember that just because a gene is expressed at a certain stage doesn't mean it is necessary for that stage to exist. Think of what we learned of B-cell development and activation, when are these genes required. 

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