Reporting results of concentration of a solution?
Hey so I have a stock solution at 5 mM concentration. I made aliqouts of this solution in water with 1 uL of stock solution in 100uL of water. I then used 5 uL of the aliquot solution in my experiment. I want to report these results and I think the correct way to do this 5uL of a 1% solution (5 mM with the name of reagent and where is was purchased). Would this be correct. Thank you!
- hcbiochemLv 74 weeks ago
No, it isn't correct. Why not just calculate and report the actual concentration of the reagent used in the experiment.
Since you diluted the 5 mM stock 100-fold, its concentration is 50 uM in the solution you actually used. So you could say "5 uL of 50 uM ____ "