Jax asked in Science & MathematicsChemistry · 1 month ago

Tris Buffer Questions?

What masses of solid Tris and Tris HCl (Tris = tris(hydroxymethyl)aminomethane, pka=8.10) should be combined and diluted to 100.0 mL to make a 0.100 M buffer with pH = 7.40

I tried using an ICE table and the Henderson-Hasselbalch equation but I kept getting a negative answer which is obviously wrong. If someone could show me the proper way to do this with the answer, it would be greatly appreciated!

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