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Anonymous asked in Science & MathematicsChemistry · 4 months ago

What is the predicted absorbance of this solution at 413 nm (λmax) when read in the Shimadzu spectrophotometers in lab?

You have a solution of 1 ml of murine neuroglobin (mNgb) that is 2.82 mg/ml according to a Bradford assay. The molar extinction coefficient at 413 nm of mNgb (ε413) is 82.1 mM-1 cm-1 and its molecular weight is 17, 600 Da).

What is the predicted absorbance of this solution at 413 nm (λmax) when read in the Shimadzu spectrophotometers in our lab?

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  • 4 months ago
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    Absorbance = ε (path length) concentration

    concentration = 2.82 g/L / 17600 g/mol = 1.60X10^-4 mol/L = 0.16 mM

    Absorbance = 82.1 mM^-1 cm^-1 (1 cm) (0.16 mM) = 13.1

    I assumed a 1 cm path length for the cell you are using. That may well not be correct if you are using a mini cell of some sort.

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