Anonymous
Anonymous asked in Science & MathematicsChemistry · 4 months ago

You need 250 ml of 3.00 μM MET-Hb final concentration in buffer A. How many mg of solid MET- Hb do you weigh out to prepare this solution?

You need 250 ml of 3.00 μM MET-Hb final concentration in buffer A. How many mg of solid MET- Hb do you weigh out to prepare this solution?

The molecular weight of the MET form of Hemoglobin tetramer is

~64,500 Da and its molar extinction coefficient at the local maxiumum (405 nm "Soret" band) is 456 mM-1 cm-1.

Update:

What absorbance reading do you anticipate from the this solution when it is prepared and read in a cuvette of 1.000 cm pathlength? What is the absorbance when read in a plate reader with 0.6 cm pathlength?

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  • 4 months ago
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    3X10^-6 mol/L X 0.250 L X 64,500 g/mol = 0.048 g = 48 mg

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