In your analysis of a quote, do you have to cite the quote again when referring to a snippet of it?

Quote: “I have a faint cold fear thrills through my veins / That almost freezes up the heat of life” (4.3.16-17).

If I want to refer to the "heat of life" in my analysis of the quote, do I have to cite it again with the act, scene, and line numbers?
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