Can you help me with the control for this experiment? (catalase enzyme)?

Question

Ok the topic is " Catalase is an enzyme which catalyses the breakdown of hydrogen peroxide into water and oxygen gas "

Some pointers: 1) i do not want to put liver in my experiment. Just like a few drops of the actual enzyme catalse"

2) i don't need anything with temperature and pH and all that ..

MY PROBLEM IS I DO NOT HAVE A CONTROL I NEED HELP IN FINDING A CONTROL.

So far what i have is:

1. Measure 10mL of 1.5% hydrogen peroxide into the measuring cylinder.

2. Pour this into the 50mL beaker.

3. Using a dropper, add 1mL of the cold, fresh catalase into the 50mL beaker containing the hydrogen peroxide.

4. Examine and record the observations.

But no control.. what to do???

Answer 1

Favorite Answer

Easy--make up a second beaker with 10 ml of peroxide in it, just like the one you described, but instead of adding catalase, add a similar volume (1 ml) of water. An even better control would be some of the same catalase which you first boil (to destroy the enzyme activity) before adding.