microscope help please!? What are the differences between iris diaphragm and condenser?
What are the differences between iris diaphragm and condenser?
- Formerly Peter SLv 76 years agoFavorite Answer
The iris diaphragm controls the amount of light passing through the slide or specimen, while the substage condenser focuses a cone of light on the slide or specimen.
- 5 years ago
The substage condenser gathers light from the microscope light source and concentrates it into a cone of light that illuminates the specimen with uniform intensity over the entire viewfield.
iris diaphragm controls the angle of illuminating rays (and thus the aperture) which pass through the condenser, through the specimen and then into the objective.
- HeatherLv 44 years ago
Eyepiece: The lens the viewer looks through to see the specimen. The eyepiece usually contains a 10X or 15X power lens. Diopter Adjustment: Useful as a means to change focus on one eyepiece so as to correct for any difference in vision between your two eyes. Body tube (Head): The body tube connects the eyepiece to the objective lenses. Arm: The arm connects the body tube to the base of the microscope. Coarse adjustment: Brings the specimen into general focus. Fine adjustment: Fine tunes the focus and increases the detail of the specimen. Nosepiece: A rotating turret that houses the objective lenses. The viewer spins the nosepiece to select different objective lenses. Objective lenses: One of the most important parts of a compound microscope, as they are the lenses closest to the specimen. A standard microscope has three, four, or five objective lenses that range in power from 4X to 100X. When focusing the microscope, be careful that the objective lens doesn’t touch the slide, as it could break the slide and destroy the specimen. Specimen or slide: The specimen is the object being examined. Most specimens are mounted on slides, flat rectangles of thin glass. The specimen is placed on the glass and a cover slip is placed over the specimen. This allows the slide to be easily inserted or removed from the microscope. It also allows the specimen to be labeled, transported, and stored without damage. Stage: The flat platform where the slide is placed. Stage clips: Metal clips that hold the slide in place. Stage height adjustment (Stage Control): These knobs move the stage left and right or up and down. Aperture: The hole in the middle of the stage that allows light from the illuminator to reach the specimen. On/off switch: This switch on the base of the microscope turns the illuminator off and on. Illumination: The light source for a microscope. Older microscopes used mirrors to reflect light from an external source up through the bottom of the stage; however, most microscopes now use a low-voltage bulb. Iris diaphragm: Adjusts the amount of light that reaches the specimen. Condenser: Gathers and focuses light from the illuminator onto the specimen being viewed. Base: The base supports the microscope and it’s where illuminator is located. Binocular Tube Holds 2 eyepieces or oculars Oculars (eyepieces) 2 Lenses positioned in binocular tube; magnifies image 10x Diopter adjustment ring Ring at base of left eyepiece; adjusted according to interpupillary distance Stand/Arm Used to carry microscope Revolving Nosepiece Revolving plate which holds objective lenses Objectives 4 lenses which magnify the image Scanning objective Magnifies 4x Low power objective Magnifies 10x High (dry) power objective Magnifies 40x Oil immersion objective (longest objective) Magnifies 100x Stage Platform containing a central opening; contains a specimen holder with a spring-loaded finger Mechanical Stage Device which holds the microscope slide in place and moves it along the stage Condenser Group of lenses below the stage in which directs and concentrates the light beam from the lamp to the specimen Diaphragm lever Adjusts the amount of light passing through the condenser; controls brightness Coarse Adjustment Knob Focuses image by raising and lowering the stage; clockwise=raises stage, counter-clockwise=lowers stage Fine Adjustment Knob Small knob in center of course adjustment knob used for final focusing Base Bottom support; houses lamp Lamp Light source; a tungsten bulb Voltage control dial or slide Controls intensity of light from lamp Power switch Switch on front which turns on lamp; indicator lamp shines when light is on
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- ?Lv 43 years ago
I think it depends