How to make 200 ml TE solution?

You want to make a TE solution, which is commonly used in molecular biology. The "recipe" for TE is 10 mM Tris-Cl and 1mM EDTA. If you have stock solutions of 1 M Tris-Cl and 0.5 M EDTA, how would you make 200 ml of the TE solution?

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  • 8 years ago
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    M1V1 = M2V2

    For Tris-Cl:

    M2 =10 mM Tris-Cl

    V2 = 200 mL

    M1 = 1 M Tris-Cl,

    V1 = unknown

    M1V1 = M2V2

    V1 = M2V2/M1

    V1 = (10 mM)*(200 mL)/(1 M)

    V1 = 2000 mmL = 2 mL

    For EDTA:

    M2 = 1 mM EDTA

    V2 = 200 mL

    M1 = 0.5 M EDTA

    V1 = unknown

    M1V1 = M2V2

    V1 = M2V2/M1

    V1 = (1 mM)*(200 mL)/0.5 M)

    V1 = 400 mmL = 0.4 mL

    So pipet 2 mL of the 1 M Tris-Cl and 0.4 mL of the 1 mM EDTA solutions into a 200-mL volumetric flask and dilute to volume with deionized or distilled water.

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