What determines how dna will be cut by a restriction enzyme?
- 7 years agoFavorite Answer
Every restriction enzyme have their own recognition site or sequence of DNA to recognize and after the recognition the site is cut by these enzymes.
for example, type I, type II and so on.
the following may help you further understand this process
- 7 years ago
It depends on the type of the restriction enzymes and in each type, on individual enzyme. There are 3 types; type II enzymes cut within the restriction site that they recognize whereas for type I & III, the restriction/recognition site and the cutting site are different.
The no. of fragments generated after treatment with a restriction endonuclease enzyme depends on the size and no. of it's restriction site present on the polynucleotide.