What is planar chromatography? Column chromatography?

What is planar chromatography? Column chromatography?

What are the differences between the two?

2 Answers

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  • ?
    Lv 4
    7 years ago
    Best Answer

    Planar chromatography

    Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin layer chromatography). Different compounds in the sample mixture travel different distances according to how strongly they interact with the stationary phase as compared to the mobile phase. The specific Retention factor (Rf) of each chemical can be used to aid in the identification of an unknown substance.

    Column chromatography

    Column chromatography is a separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open tubular column). Differences in rates of movement through the medium are calculated to different retention times of the sample.

    For more details

    http://en.wikipedia.org/wiki/Chromatography

  • hoggan
    Lv 4
    3 years ago

    Planar Chromatography

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