How do you count bacterial colonies using the pour plate method?
Do you count only the colonies growing on the surface of the agar for total viable bacterial count, or do you also count those anaerobic bacteria embedded in the agar? If so, wouldn't it be hard to do so?
- hcbiochemLv 78 years agoFavorite Answer
If your bacteria are facultative anaerobes, they will grow both on the surface and within the agar. Anarobes will grow within the agar but not on the surface. To do a total viable count, you should count ALL colonies in and on the agar.