Organic acid derivatization?
I am trying to analyze Sodium Acetate Trihydrate, Proponic Acid, Gluconic Acid and Sodium Octanoate by GC-TCD. I prepared my organic acid standards with methanol( 3%v/v Sulfuric acid) and chloroform which contains benzoic acid as internal standard. Then I digested them at 100C for 2 hours. With adding DI water I separate organic acid phase ( which is in chloroform) and put it into GC vials. I set up my oven temperature both at 40C and 125C ( two different methods), but I am not able to get any peaks for standards. I have done with literature review, but unfortunately I ended up with the method for urine. Does anyone give me some advice about my method or any other method?
I really need help as soon as possible.
- Sam SaunLv 68 years agoFavorite Answer
You should read the link because it seems that your thermal conductivity detector is not compatible with acids, halogenated organics, and oxidizing agents (e.g. oxygen). Since you did load all of the above into the GC, I think you might have killed the wires of the detector. You will need to change that and avoid using acids with it.
- hanterLv 44 years ago
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