Hi asked in Science & MathematicsBiology · 8 years ago

recombinant dna technology?

what is rdt

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  • Daany
    Lv 5
    8 years ago
    Favorite Answer

    recombinant DNA tech basically means that you use a cloning vector from a bacteria to replicate a desired gene. So, for example, when you want multiple copies of a certain gene for an experiment you would use this technique. Also, this is how bacteria can be 'tricked' into reproducing proteins and other compounds that humans use, like insulin. It's important because bacteria replicate quickly so you can get lots of copies of a gene in a small period of time.

    The simplest organism to do this in is E. Coli, and other prokaryotes like E. Coli have a plasmid vector which is a circular DNA molecule in addition to the rest of their genetic material. The way it works is that the plasmid vector is isolated, and digested with restriction enzymes that cleave the DNA at a known sequence. Then, you pick the gene that you want to replicate and use the same restriction enzyme to cleave it. Now you have ends that can be hybridized together, so you 'glue' the gene of interest into the plasmid vector with DNA ligase. Then you allow it to be taken up into the bacteria (called 'transformation') and the bacteria will replicate it like their own DNA and when you have lots of bacteria you can isolate the gene again and have lots of copies of it.

    Of course, not all bacteria have plasmids so you 'select' bacteria that did take up the DNA by growing it on a solid medium with an antibiotic like ampicillin. This will kill off all bacteria without plasmids because the antibiotic resistance genes of a bacterium are found in the plasmid, so only those with the gene survive.

    There's more to it, but that's it in a nutshell :)

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  • Anonymous
    8 years ago

    It's how most of vaccines are made. IT's use the cross-immunity of immune memory to it's advantage. Foreg. Cow pox doesn't affect humans but it's virus can provide immune memory for chicken pox that affects humans. They use an antigen that doesn't affect humans and grow it in suitable medium and then store the live culture in vaccine.

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  • Nnj
    Lv 4
    8 years ago

    Dono

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