Reverse and forward primers are complementary to each other, in their presence during PCR how we can avoid dim?
When we are doing PCR we use reverse and forward primers during bacterial identification which are complementary to each other (whatever these may be universal or specific primers) then how we can avoid dimer formation? if through high temperature what will be the temperature?
- EllieLv 49 years agoFavorite Answer
High temperatures are used to dissociate primers from complementary DNA in order to allow the next round of replication. The temperature then has to be decreased to allow the primer to anneal to DNA. If your reverse and forward primers are totally complementary then you can't prevent primer dimer formation whilst allowing the primers to anneal to the target DNA. However a certain amount of complementarity can be managed using higher annealing temperatures. It is important however that the primer has a higher degree of complementation with the target DNA than with it's dimer partner to allow it to anneal to preferentially anneal to the target DNA.Source(s): Years of doing PCR
- AbuLv 69 years ago
They are not complementary to each other.