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Anonymous asked in Science & MathematicsBiology · 1 decade ago

how do you know when you have reached optimal pH for an enzyme?

Also how do you convert absorbance per second to absorbance per minute?

3 Answers

  • 1 decade ago
    Favorite Answer

    im going to assume ur using a spectrometer to observe change in Abs per second caused by a product of the enzymatic activity. while substrates are much greater than the enzyme, ergo it it is running at its Kmax for that pH.

    the greater the change per second the faster it is working.

    if u repeat this experiment using various pH and plot pH vrs Abs/Sec, then it'll form a peak somewhere--or if u did it all at once where u slowly changed the pH, then it would be the steepest part of the Abs/time graph... but.. most spectrometers aren't that fancy. at least not he ones that they let students work with.

    also yes. multiply by 60.

    (comon, bro, u're prob in college right?)

    Source(s): 4rth year microbio major. research assistant in molecular bio lab
  • BP
    Lv 7
    1 decade ago

    The enzyme will work faster at optimal pH, than at any other pH lower or higher than the optimal.

    Multiply by 60

    Source(s): bp
  • 1 decade ago

    At first i tried to know about it's symptoms, than went to hospital.

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