Growing HeLa Cells in CO2 Independent Medium?
For all those experienced in biology and cell culture, do you think it would be possible to successfully grow HeLa cells in Gibco (Invitrogen) CO2 independent medium in a regular 37 degree incubator? Our research facility does not have access to a 5% CO2 incubator, so we are looking for ways to work around this to grow our HeLa cells. If you know of any other efficient methods for growing these cells without CO2, all help is much appreciated. Thanks again :D
- misoma5Lv 710 years agoFavorite Answer
Unfortunately, these cells will still require what they are used to in their natural environment. 37C, 5% CO2 for mammalian cells.Source(s): MD
- blomquistLv 43 years ago
definite, that's noted as "tissue custom" and diverse varieties of cells are used this way for loads of examine purposes. here is extra archives from a molecular biology type description.
- 10 years ago