Growing HeLa Cells in CO2 Independent Medium?

For all those experienced in biology and cell culture, do you think it would be possible to successfully grow HeLa cells in Gibco (Invitrogen) CO2 independent medium in a regular 37 degree incubator? Our research facility does not have access to a 5% CO2 incubator, so we are looking for ways to work around this to grow our HeLa cells. If you know of any other efficient methods for growing these cells without CO2, all help is much appreciated. Thanks again :D

4 Answers

  • 9 years ago
    Favorite Answer

    Unfortunately, these cells will still require what they are used to in their natural environment. 37C, 5% CO2 for mammalian cells.

    Source(s): MD
    • Login to reply the answers
  • ortuno
    Lv 4
    3 years ago

    Co2 Independent Medium

    • Login to reply the answers
  • 3 years ago

    definite, that's noted as "tissue custom" and diverse varieties of cells are used this way for loads of examine purposes. here is extra archives from a molecular biology type description.

    • Login to reply the answers
  • 9 years ago


    • Login to reply the answers
Still have questions? Get your answers by asking now.