Yahoo Answers is shutting down on May 4th, 2021 (Eastern Time) and the Yahoo Answers website is now in read-only mode. There will be no changes to other Yahoo properties or services, or your Yahoo account. You can find more information about the Yahoo Answers shutdown and how to download your data on this help page.

Anonymous
Anonymous asked in 科學化學 · 1 decade ago

麻煩幫我翻譯一下 Materials and methods

2.2. Microorganism and fermentation

Bacillus subtilis natto (ATCC 7059) was first cultured in nutrient

broth (NB) flasks from a stock culture maintained in nutrient agar

slant. Seed culture of Bacillus subtilis natto was prepared by trans-

ferring 0.1% of bacteria from the NB flask into a Erelenmeyer flask

(300 mL) containing 100 mL of NB medium and incubated in shaker

at 37 ◦C, 120 rpm for 12 h. The fermentation medium containing 10 g

Astragalus membranaceus var. mongholicus powder and 90 g water

was inoculated with 0.1% of the seed culture and then cultivated at

37 ◦C, 120 rpm for 48 h (HQB). As the control, the 10% Radix astra-

gali medium was also incubated at the same condition without

inoculation (HQNB). After fermentation or incubation, the broth

was centrifuged and the supernatant was filtered, freeze dried, and

stored at −20 ◦C for further experiments. All fermentation experi-

ments were performed in triplicate to ensure the reproducibility.

Normally, the yield was around 29%.

2.3. Cell culture

Primary cultures of normal human epidermal keratinocytes

(HEK, Cat. C-013-5C) and normal human dermal fibroblasts (HDF,

Cat. C-013-5C), derived from adult skin were purchased from Cas-

cade Biologics Company (Portland, OR). They were cultured in

EpiLife medium (Cat. M-EPIcf-500) with Human Keratinocytes

Growth Supplement (HKGS, Cat. S-001-5, Cascade Biologics) and

in DMEM medium (Invitrogen Life Technologies Inc., USA) with

10% FBS, respectively. In some experiments, the HaCaT cell line

(Boukamp et al., 1988), spontaneously transformed keratinocytes

purchased from Cell Lines Service (Germany) and maintained in

DMEM medium supplemented with 10% FBS, was used instead of

normal keratinocytes. Both keratinocytes and fibroblasts were cul-

tured in a humidified incubator at 37 ◦C under an atmosphere of

95% air and 5% CO2.

Update:

不要只用翻譯軟體的給我好嗎...請化學相關的大大們給些正確的名詞解釋~"~...

翻譯軟體的有限...

1 Answer

Rating
  • 1 decade ago
    Favorite Answer

    2.2。微生物發酵

    納豆芽孢桿菌(ATCC 7059)首次在營養培養

    肉湯(注)燒瓶從股票的文化保持營養瓊脂

    傾斜。種子培養納豆枯草芽孢桿菌製備了跨

    輝凌0.1%的細菌,毒品調查科瓶瓶成Erelenmeyer

    (300毫升)含100毫升中,毒品調查科在培養搖床

    在37◦葷,120每小時轉 12發酵培養基中的10克

    黃芪。黃芪粉和90克水

    接種 0.1%的種子培育文化,然後在

    37◦葷,120轉 48小時(衛生檢疫)。作為對照,10%的基數阿斯特拉-

    加利還培養基培養在相同條件下無

    接種(HQNB)。經過發酵或培養,發酵液

    離心的上清液過濾,凍乾,和

    儲存在-20◦C的進一步試驗。所有發酵 experi -

    分別進行發言:一式三份,以確保重複性。

    通常情況下,收益率約為 29%。

    2.3。細胞培養

    原代培養正常人表皮角質形成細胞

    (引領,貓。ç - 013 - 5C型)和正常人皮膚成纖維細胞(高密度纖維板,

    貓。 ç - 013 - 5C型),來自成人皮膚購自栲

    凱德生物製品公司(波特蘭)。他們培養

    EpiLife介質(列寧博物館的M - EPIcf - 500)與人角質形成細胞

    生長補編(HKGS,貓。第S - 001 - 5,級聯生物製劑)和

    在DMEM培養液(Invitrogen公司生命科技公司,美國),

    10%胎牛血清,分別為。在一些實驗中,角質形成細胞株

    (Boukamp等。,1988),角質形成細胞自發轉化

    細胞系購買服務(德國)和保持

    培養基培養基含10%胎牛血清,而沒有使用

    正常角質形成細胞。這兩個角質形成細胞和成纖維細胞,小路

    tured在加濕孵化器在37◦C溫度條件下的氣氛中

    95%的空氣和5%的二氧化碳

Still have questions? Get your answers by asking now.