1. The depectinized juice was then clarified with 6 ml of 5% bentonite and 5 ml
of 15% kizelsol at 50℃ for 30 min.
2.For this purpose, aliquots of fruit juice matrices containing black carrot
concentrate were brought to pH 1.0 and 4.5 and allowed to equilibriate for 1 h.
3.The absorbance of each equilibrated solution was then measured at the
wavelength of maximum absorption (kmax) and 700 nm for haze correction,
using a ThermoSpectronic Helios-a model spectrophotometer .
4.The difference in absorbance values at pH 1.0 and 4.5 was directly
proportional to total anthocyanin concentration which was calculated, based
on cyanidin-3-glucoside with a molecular weight of 445.2 and molar
absorbance of 29,600.
- 1 decade agoFavorite Answer
1 3 2 4
For this purpose
The absorbance...was then
The difference...at pH 1.0 and 4.5