Evie asked in 科學化學 · 1 decade ago

20點!!急呀!Agarose 的分類?不同EEO有何功能?

各位大大:

實驗室常常使用的Agarose有很多不同的種類,我想請問,有哪位大大知道各種不同條件的agarose分別有何功能?應用在何處呢?都用來做什麼樣的實驗呢??

1. low-EEO, mid-EEO,high-EEO Gel

2. low melting point, mid milting point, hight milting point

3. low gelling temperature, mid gelling temperature, high gelling temperature

希望能有專業,詳細且完整的說明!

1 Answer

Rating
  • 小陳
    Lv 6
    1 decade ago
    Favorite Answer

    讓戰將來回答您~~

    1. low-EEO, mid-EEO,high-EEO Gel

    low-Electroendosmosis (EEO) - 指核酸與膠體有較低的結合力, 通常 low-EEO 有較高解析度, 可適用於 restriction digest fragment Southern, Northern 等實驗!

    2. low melting point, mid milting point, hight milting point

    低熔點的膠用來方便回收電泳後的 DNA 片段

    高熔點的我就沒用過了~~

    3. low gelling temperature, mid gelling temperature, high gelling temperature

    gelling temperature= melting point, 解釋如上面第2點

    希望以上回答有幫到您~~

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