皮皮兒 asked in 科學化學 · 1 decade ago

Western blotting之TBST配法

0.1% TBST?

0.05% TBST?

Update:

2%BSA/ 0.05%TBST

如何配..

1 Answer

Rating
  • 小陳
    Lv 6
    1 decade ago
    Favorite Answer

    讓戰將來回答~~

    圖片參考:http://www.molecularstation.com/images/western-blo...

    0.05%TBST

    1. Pour 3 L of purified water into a 4-L beaker and begin stirring.

    2. Measure 400ml 10X TBS in a 500-ml graduated cylinder and add to water.

    3. Add BSA and 2ml Tween 20 to the beaker. Rinse all of the Tween 20 from the pipette tip into the solution.

    4. Transfer solution to a graduated cylinder and adjust to final volume 4L with purified water.

    5. Return solution to beaker and stir until thoroughly mixed.

    以上是以 4L 為配置體積, 若你用不到那麼多可依比例減少配置體積, 而 2%BSA 的配法則依此要領, 將你們實驗室中用的 BSA 加入即可, 因為不知道你們是用 powder 還是 liquid 的 BSA~

    圖片參考:http://www1.qiagen.com/Products/GeneSilencing/AllS...

    希望以上有幫到您~~

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