Anonymous asked in Science & MathematicsMedicine · 1 decade ago

Do any of you know about Quintos and Santos in the field of bacterial paper discs and its level of activation?

it's about reading assay plates in our research's methodology..

1 Answer

  • 1 decade ago
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    "There are many good tests available but the limiting factor is always cost and the expertise you need,"


    With the paper-disc method, a broth containing the bacteria can be prepared, packed on ice and taken to the field for on-site water tests. One small Coca-Cola bottle full of prepared liquid is enough for more than 1,000 water samples, and the test can be carried out with a minimum of training.

    For Quinto, it is deeply satisfying that he didn't have to rely on lab products from the West for his method. "There are so many bio-resources that can be tapped here in the Philippines," he says. He talks about the local reluctance to use homegrown methods, though they're often cheaper. His adviser, Fortunato Sevilla agrees: "Maybe it's the colonial experience we have. We'd rather have something foreign than something locally made."

    Quinto has high hopes for other uses for his bacteria cultures, for example, to test urine for, say, mercury poisoning. Or for use by any company that needs to test its products for antibacterial activity. Meanwhile, a certain group of luminous bacteria has become a problem as they have infested prawn farms and Quinto says that in a sort of role reversal, a plant extract that has been shown to harm the bacteria could be used as a natural pesticide.

    Quinto also wants to use the method to test indigenous plants for medical properties. Traditionally, it takes lots of petri dishes and long labour as well as vast quantities of samples to see whether a plant may have wonder-drug potential, but with the paper-disc test, only two or three leaves from each plant are necessary to give a quick indication of its powers. Bacteria exposed to garlic lose their glow, for example, indicating possible antibacterial properties.

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