女仔 asked in 科學及數學化學 · 1 decade ago

Real Time PCR (chain reaction)

Why is PCR important? WHat are the uses of it?

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  • nick
    Lv 5
    1 decade ago
    Favorite Answer

    What is pcr?

    PCR is used to cut the dna into shorter fragment, in a particular length.

    In pcr, polymerase chain reaction, we use a enzyme, polymerase to cut and copy dna to amplify a dna sample from dna extraction. We will also use magnesium chloride, forward and reverse primer etc. We will need to run the reaction at different temperature for different times to produce the largest amount dna.

    Why is PCR important?

    The dna we obtained from extraction are little in amount that we can't run it on the gel electrophoresis. On the other hand, the dna fragment is too large to pass through the gel, the result we will see is just a messy stuff.

    What are the uses of it?

    As the dna we want a painless dna collection and the dna we collect may not be enough to run in a gel electrophoresis, we have to use the pcr to amplify the dna

    Source(s): 此文章為本人作答,未經許可,不得轉載!
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