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拜託!!有關草本植物的期刊....有誰可以幫忙翻譯
HOCl or H2O2-luminol chemiluminescence.
A schematic diagram of the FIA-CL system is shown in Fig. 1.
The peristaltic pump was a Gilson Minipuls 3 and
the injection valve was a Rheodyne RH-5020, obtained
from Anachem (Luton, Bedfordshire, UK).
The pump tubing had a suitable internal diameter to deliver
the required flow-rate. The remainder of the flowinjection
manifold was constructed from PTFE tubing
joined with low-pressure fittings from Anachem
(UK).
CL detection was carried out using a luminometer
(Model: Lumi-Flo, Chrono-log, USA). The
results were recorded on a chart recorder (Model
706-707, Chrono-log, USA).
The oxidant stream was merged with a luminol/buffer
reagent immediately before the luminometer. The total
flow rate was 1 mL/min, shared equally between the
luminol and the oxidant channel; the oxidant channel
includes an injection valve in the middle allowing successive
nominal 20 μL injections of the plant extracts
to be made. Mixed flow of oxidant/antioxidant was
matched with luminol/buffer before the entrance to the
flow cell.
拜託了..
1 Answer
- 1 decade agoFavorite Answer
HOCl或H2O2 -魯米諾發光。
示意圖國際汽聯發光系統中顯示圖。 1 。
在蠕動泵是一個吉爾松Minipuls 3
注射閥是Rheodyne濕度- 5020 ,獲得
從Anachem (盧頓,貝德福德郡,英國) 。
泵油管有合適的內部直徑提供
所需要的流量。其餘的flowinjection
從多方面構建聚四氟乙烯管
一道低壓配件從Anachem
(英國) 。
發光檢測進行了使用luminometer
(型號:河,弗洛,時辰日誌,美國) 。那個
結果記錄在圖表記錄器(型號
706-707 ,時辰日誌,美國) 。
氧化劑流合併與魯米諾/緩衝區
試劑前夕luminometer 。總
流速為1毫升/分鐘,平均分擔
魯米諾和氧化劑通道;氧化劑頻道
包括一個噴射閥在中東允許連續
標稱20 μL注入的植物提取物
必須作出。混流的氧化/抗氧化是
符合魯米諾/緩衝區之前入口
流動細胞。
Source(s): 誰