小雨文 asked in 科學植物學 · 1 decade ago

拜託!!有關草本植物的期刊....有誰可以幫忙翻譯

HOCl or H2O2-luminol chemiluminescence.

A schematic diagram of the FIA-CL system is shown in Fig. 1.

The peristaltic pump was a Gilson Minipuls 3 and

the injection valve was a Rheodyne RH-5020, obtained

from Anachem (Luton, Bedfordshire, UK).

The pump tubing had a suitable internal diameter to deliver

the required flow-rate. The remainder of the flowinjection

manifold was constructed from PTFE tubing

joined with low-pressure fittings from Anachem

(UK).

CL detection was carried out using a luminometer

(Model: Lumi-Flo, Chrono-log, USA). The

results were recorded on a chart recorder (Model

706-707, Chrono-log, USA).

The oxidant stream was merged with a luminol/buffer

reagent immediately before the luminometer. The total

flow rate was 1 mL/min, shared equally between the

luminol and the oxidant channel; the oxidant channel

includes an injection valve in the middle allowing successive

nominal 20 μL injections of the plant extracts

to be made. Mixed flow of oxidant/antioxidant was

matched with luminol/buffer before the entrance to the

flow cell.

拜託了..

1 Answer

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  • 1 decade ago
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    HOCl或H2O2 -魯米諾發光。

    示意圖國際汽聯發光系統中顯示圖。 1 。

    在蠕動泵是一個吉爾松Minipuls 3

    注射閥是Rheodyne濕度- 5020 ,獲得

    從Anachem (盧頓,貝德福德郡,英國) 。

    泵油管有合適的內部直徑提供

    所需要的流量。其餘的flowinjection

    從多方面構建聚四氟乙烯管

    一道低壓配件從Anachem

    (英國) 。

    發光檢測進行了使用luminometer

    (型號:河,弗洛,時辰日誌,美國) 。那個

    結果記錄在圖表記錄器(型號

    706-707 ,時辰日誌,美國) 。

    氧化劑流合併與魯米諾/緩衝區

    試劑前夕luminometer 。總

    流速為1毫升/分鐘,平均分擔

    魯米諾和氧化劑通道;氧化劑頻道

    包括一個噴射閥在中東允許連續

    標稱20 μL注入的植物提取物

    必須作出。混流的氧化/抗氧化是

    符合魯米諾/緩衝區之前入口

    流動細胞。

    Source(s):
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