cindy asked in 社會與文化語言 · 1 decade ago

B肝病毒DNA的PCR

Although classical PCR-based assays have increased the

sensitivity of analyses, their application in clinical practice

is hindered by technical complexity, susceptibility to

contamination and variable reproducibility . Recent advances in PCR

technology allow continuous measurement of the fluorescence

emitted during amplicon production after each PCR

cycle. This is achieved using a variety of different fluorescent

chemistries that correlate PCR product concentration

to fluorescence intensity .

Reactions are characterized by the time point (or PCR

cycle) where target amplification is first detected. This

value is usually referred to as the cycle threshold (Ct) or

crossing point (Cp), the time at which fluorescence intensity

is significantly greater (usually ten times the SD of the

baseline) than background fluorescence (F0). Consequently,

the greater the quantity of target DNA in the starting

material, the faster a significant increase in fluorescence

signal will appear, yielding a lower Ct

There are many benefits of using real-time PCR over

other methods to quantify target DNA.

1 Answer

Rating
  • book
    Lv 5
    1 decade ago
    Favorite Answer

    1.Although classical PCR-based assays have increased the sensitivity of analyses,

    雖然以PCR為基礎的傳統標準分析化驗報告,已經把各種分析研究的

    (細微精密性)/敏銳準確度大大地提高了,

    their application in clinical practice但他們在臨床的實際應用上

    is hindered by technical complexity現在還是被技術層面上的複雜性所阻礙,

    susceptibility to contamination .(以及)...操作過程對污染物的不穩定性(操作過程易受到污染物的影響.容易產生不穩定性的結果)

    and variable reproducibility

    並且(會產生)容易改變性質的有可再繁殖力(的子世代).

    Recent advances in PCR technology有關PCR技術的最近的發展..已經進展到可以容許對 被發出的螢光做持續性的測量,

    allow continuous measurement of the fluorescence emitted

    during amplicon production 在大量合成產物的期間

    after each PCR cycle. 在每一個PCR的使用週期之後,

    This is achieved 這using a variety of different fluorescent chemistries

    使用各種不同的螢光劑(產生的)化學作用 的技術也被達成,

    that correlate PCR product concentration

    這項技術關聯到PCR的產物的集中程度

    to fluorescence intensity .對於螢光的強度

    Reactions are characterized by the time point (or PCR cycle)

    這些化學反應具有以..某特定時間點(or PCR cycle或是PCR週期)

    為特徵

    where target amplification is first detected. 在這裡.....(以螢光的強度來標定產生物的量與質).....目標產物首度被(以用螢光方式)測定出來,

    This value is usually referred to as the cycle threshold這好處就是..通常.(可以從螢光的程度判定出PCR反應程度)...是(到了)週期;循環;起始點 (Ct) or或是 crossing point (Cp),終點(Cp),

    the time at which fluorescence intensity在(Ct)起點或是(Cp)終點,的時間點,

    螢光的強弱程度(之差異) is significantly greater usually ten times the SD of

    the baseline通常基底線的螢光度SDthan background fluorescence (F0)大於背景的螢光度十倍.

    Consequently,結果

    the greater the quantity of target DNA in the starting material,

    在初始原料方面..對產生目標DNA的數量.產生量愈多.....

    the faster a significant increase in fluorescence signal will appear, 螢光顯示度很明顯的增加情況---將會愈快出現.

    yielding a lower Ct 同時生產出比較低量的 Ct

    There are many benefits of using real-time PCR over other methods to quantify target DNA.*

    使用實際(立即反應的) real-time PCR 有許多好處... (遠甚於)超過~使用別的方法,去大量生產目標物DNA.

Still have questions? Get your answers by asking now.