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Anonymous
Anonymous asked in 科學生物學 · 1 decade ago

塗佈培養和注入培養的問題 急~

請問有誰能告訴我『塗佈培養』和『注入培養』各別的優缺點和用途,以及分別適合何種菌種、有哪些菌?

2 Answers

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  • 1 decade ago
    Favorite Answer

    塗佈培養(spread plate):將菌液以susan table或玻璃珠塗佈在固體培養基上

    優點:操作方便能快速分辨出單一菌落

    缺點:容易污染保存不易

    用途:挑選單一菌落

    注入培養(pour plate):將菌液經過一系列稀釋後,取適量與培養基混勻再倒入培養皿中

    優點:較不易污染

    缺點:操作麻煩

    用途:挑選單一菌落,計算生菌數

    合適菌種要看培養基的成分決定

    Source(s): 微生物實驗筆記
  • 1 decade ago

    塗佈培養-就是將菌種以塗抹或劃法均勻在培養皿agar.至於溫度時間各菌種不一樣.

    優點--容易判定.純化菌種.收菌方便菌數高.

    缺點--易污染.

    注入培養--將菌以液體培養方式.把菌種少量注入到大量培養液培養.

    優點--比較方便

    缺點--菌數少.空間需較大.

    Source(s): 自己經驗
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