Materials and methods
2.1. Plant material
Salvia miltiorrhiza flower was collected at seven locations in China
in 2006 (Table 1). All samples were air-dried at room temperature
in a shady place and kept from direct light.
Hydrodistillation was used for 6 h to extract the essential oils.
Distillate was extracted with ether, and then frozen at 18 C to remove
2.2. GC analysis
Analytical gas chromatography was carried out using a Shimadzu
(Osaka, Japan) GC-2010A series gas chromatograph with an
OV-1701 capillary column (30 m 0.32 mm, 0.33 lm film thickness).
Nitrogen was the carrier gas. Column temperature was initially
kept at 70 C for 5 min, then gradually increased to 280 C
at a rate of 5 C/min, and held for 10 min. Samples of 1 ll were injected
at 280 C, manually with split mode. Flame ionisation detection
(FID) was set at 280 C.
2.3. GC–MS analysis
GC–MS analysis was performed on a 6890N gas chromatograph
with fused silica capillary column (J&W HP-5, 30 m 0.25 mm,
0.25 lm film thickness), coupled to a mass selective detector
(MSD5973N, ionisation voltage 70 eV; all Agilent, Santa Clara,
CA). Helium was used as carrier gas. Oven temperature was from
80 C to 290 C at 4 C/min and held at 290 C for 30 min. The components
were identified by comparison of their mass spectra with
those of the NIST02L mass spectral library.
- bin0428Lv 41 decade agoFavorite Answer
氣相層析質譜儀主要用6890N氣相層析儀執行；並用玻璃纖維的毛細管柱(J&W廠牌 HP-5型號，30 米 0.25 mm口徑，0.25um 膜厚)，連接質譜儀( 質譜儀5973N型，離子化電壓70eV；均為安捷倫廠牌，美國加州聖克拉拉)，氦氣作為載氣，烘箱溫度從80℃以每分鐘升4℃至290℃並維持30分鐘。樣品組成份鑑定則是利用NIST02L質譜圖庫作為判別比對樣品質譜。
2009-02-27 13:47:01 補充：
2009-03-04 23:56:21 補充：
專討喔!加油吧! ^O^Source(s): GC