林漢祥 asked in 科學生物學 · 1 decade ago

生物~生物技術概論~shotgun cloning??

shotgun cloning

課本上翻譯為散彈選殖

請簡單幫我說明

看了好幾遍

我還是不懂這是做什麼的??

麻煩簡單說明就好

謝謝

Update:

尤其是跟基因體基因庫究竟是什麼關係呢?

這段我老是唸不懂

我再找時間把課文PO上來好了

2 Answers

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  • C C
    Lv 6
    1 decade ago
    Favorite Answer

    shotgun cloning is basically a way to break apart your DNA of interest, and then store them in vectors (usually plasmids in bacterial cells) so that you can "clone" them. The bacterial cells would then be referred to as the DNA library (I suppose, the 基因庫).

    Let's say you're trying to store the human genome in a DNA library (this is actually how the human genome is sequenced back in the late 1990s). You take the human genomic DNA, fragment it by digesting with restriction enzyme or physcially breaking it apart (through sonication). This will cause the DNAs to be fragmented into small pieces. Then there is a procedure for these DNAs to be taken up into a vector, and stored in bacterial cells. This way you will have "cloned" the pieces of DNA. It is called a 'shotgun' approach because you randomly break the DNA apart and store them. If you have many copies of the DNA, then each part of the genome will be stored in multiple bacterial cells, so you will still have a complete genome (you will not lose any DNA material).

    In sequencing the human genome, the scientists then take the DNA library, sequence the part of the plasmid they know is not bacterial, and then try to piece back together the human genome.

    You can look at this website for some basic introduction of shotgun cloning:

    http://www.iscid.org/encyclopedia/Shotgun_Cloning

    Does this make sense?

    You can try to post the original text... but I'm not sure how much I would understand if it is in Chinese...

    Source(s): I'm a graduate student in genetics.
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  • 1 decade ago

    通常還是去找原文書的原文部分再自己念比較好

    翻譯的通常都是越翻越模糊,念原文書還比較輕鬆

    我似乎學過基因槍gene gun原先是植物基因轉殖技術之一

    將要植入的DNA以金屬粒子包覆(保護)好

    像似子彈一樣利用高壓打入細胞內

    如果是shotgun cloning的話我會理解成任意選殖

    shotgun當形容詞用有任意,漫無目的的意思

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