- C CLv 61 decade agoFavorite Answer
shotgun cloning is basically a way to break apart your DNA of interest, and then store them in vectors (usually plasmids in bacterial cells) so that you can "clone" them. The bacterial cells would then be referred to as the DNA library (I suppose, the 基因庫).
Let's say you're trying to store the human genome in a DNA library (this is actually how the human genome is sequenced back in the late 1990s). You take the human genomic DNA, fragment it by digesting with restriction enzyme or physcially breaking it apart (through sonication). This will cause the DNAs to be fragmented into small pieces. Then there is a procedure for these DNAs to be taken up into a vector, and stored in bacterial cells. This way you will have "cloned" the pieces of DNA. It is called a 'shotgun' approach because you randomly break the DNA apart and store them. If you have many copies of the DNA, then each part of the genome will be stored in multiple bacterial cells, so you will still have a complete genome (you will not lose any DNA material).
In sequencing the human genome, the scientists then take the DNA library, sequence the part of the plasmid they know is not bacterial, and then try to piece back together the human genome.
You can look at this website for some basic introduction of shotgun cloning:
Does this make sense?
You can try to post the original text... but I'm not sure how much I would understand if it is in Chinese...Source(s): I'm a graduate student in genetics.
- 1 decade ago