Anonymous
Anonymous asked in 社會與文化語言 · 1 decade ago

急問,這一段話意思~有關paper

請問大師悶這段話在說明什麼 ?總結其大意即可

謝謝了 >"<

We using defective Tat-minus HIV-1 mutants, we identified the Ebola virus (EBOV) VP35 protein as a potent RNAi suppressor that is functionally equivalent to the HIV-1 Tat RSS function.

Furthermore, we show that the E3L and NS1 proteins can also functionally complement the Tat RSS function and rescue virus production of the HIV-1 Tat-minus mutants. These data support the role of RNAi in innate antiviral responses in mammalian cells and the essential role of RNAi suppression in HIV-1 replication.

Update:

These virus-specific dsRNAs are processed into small interfering RNAs (siRNAs; a 21-nucleotide dsRNA duplex) by the RNAse III–like endonuclease-denoted Dicer.

Update 2:

Subsequently, one strand of the siRNA duplex, the guide-strand,

is incorporated into the RNA-induced silencing complex (RISC) to target viral mRNAs bearing complementary sequences for destruction.

2 Answers

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  • Anonymous
    1 decade ago
    Favorite Answer

    These virus-specific dsRNAs are processed into small interfering RNAs (siRNAs; a 21-nucleotide dsRNA duplex) by the RNAse III–like endonuclease-denoted Dicer.

    這些 特殊的 dsRNAs 利用RNAse III 介入siRNAs; 中 

    Subsequently, one strand of the siRNA duplex, the guide-strand,

    is incorporated into the RNA-induced silencing complex (RISC) to target viral mRNAs bearing complementary sequences for destruction.

    其中主導的一條(one strand of the siRNA ) 介入RNA 造成”沉默的複合體 侵入病毒的mRNA 中 造成RNA序列互補的破壞  ^_^

  • Anonymous
    1 decade ago

    這段話的意思:

    我們使用瑕疵Tat減HIV-1突變體,我們辨認了埃博拉病毒(EBOV) VP35蛋白質作为是功能上相同的與HIV-1 Tat RSS作用的一臺有力RNAi遏抑器。此外,我們表示, E3L和NS1蛋白質可能功能上也補全HIV-1的Tat RSS作用和搶救病毒生產Tat減突變體的。这些數據支持RNAi的角色在固有抗病毒反應在哺乳细胞和RNAi鎮壓的根本角色在HIV-1複製。這些病毒具體dsRNAs被處理成小干涉的RNAs (siRNAs; 21核苷酸dsRNA套樓公寓)由III像核酸內切酶表示的Dicer的核糖核酸酶。随后, siRNA套樓公寓,指南子線的一條子線,被合併到核糖核酸導致的沉默的複合體(RISC)里瞄準負擔破壞的病毒mRNAs補全序列。

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