asked in 科學化學 · 1 decade ago

磷酸buffer配置PMS問題

以0.1 M phosphate buffer (pH 7.4)配製120μM之PMS

這個藥怎麼配阿??

很多paper都只有這樣一句話而已

請大家教教我

1 Answer

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  • 1 decade ago
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    Phenazine methosulphate (PMS)中文名稱: 5-甲基吩嗪硫酸甲酯. CAS號: 299-11-6. 分子式: C14H14N2O4S. 分子量: 306.34

    Promega 有賣0.92 mg/mL 的 濃縮Stock 0.92/306.34 X 10 9次方=3X10 6次方 uM

    要配成 120uM PMS in PBS (500CC)要加多少STOCK?

    120X500=3X10 6次方 X L

    L=0.02 Liter =20mL

    泡50 mL只要 2CC PMS加到PBS中即可

    10mL 400uL

    1mL=40uL

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