水蜜桃 asked in 社會與文化語言 · 1 decade ago

一段英文..請高手幫我翻譯

The thiamine (B1) and ribflavin (B2) standards were acquired from Sigma Chemical Co., USA. The standard vitamin solutions were prepared in 0.1 N HCl. The enzyme takadiastase was acquired from Fluka, Switzerland. The organic used as the mobile phase for liquid chromatography were of chromatographic grade and the other reagents of analytical grade. Water purifed in the Milli-Q(Millipore) system was used to prepare the mobile phases.All mobile phases were filtered through membranes with a pore size of 0.45 um.

An Edwards model Super Modullyo freeze-dryer was employed to freeze-dry the sample used in the validation of the methodology. An HP model 1100 high performance liquid chromatograph was used in the vitamin determinations,determinations,equipped with a degasser, quaternary pump, automatic injection system (0–100 uL), with a diode array and fluorescence detectors coupled in series, and a temperature control compartment for the analytical column. The detection system allowed for the simultaneous detection at various wavelengths. The overall system was controlled by the HP-Chemstation software, which also administered the data collection and treatment system.

The evaluation parameters used to validate the methodology for the determination of vitamins B1 and B2 were linearity,the detection and quantification limits and repeatability. All the analyses were carried out using the sample of freeze-dried A. bisporus.

Linearity was determined by constructing calibration curves with standard solutions containing the vitamins in the mass range from 1.1 to 210 ng and from 2.3 to 202 ng for vitamins B1 and B2 respectively. Three injections were made at each level. The oxidation reaction of thiamine to thiochrome was the same used on the samples analysis and was carried out as described in Section 2.5.

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請勿用翻譯軟體...那個我有了...翻出來一模一樣...

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  • Anonymous
    1 decade ago
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    該硫胺素( B1 )和ribflavin (素B2 )標準,從收購西格瑪化工有限公司,美國。維生素標準的解決方案是準備在0.1 ñ鹽酸。酶takadiastase獲得來自藍,瑞士。有機用來作為流動相的液相色譜是色譜級和其他分析試劑級。水purifed的米- Q報表(純水)系統是用於編寫移動phases.All移動相過濾膜的孔徑0.45嗯。

    愛德華茲模型的超級Modullyo冷凍乾燥機是採用冷凍乾燥的樣品中使用的驗證方法。惠普模型1100高效液相色譜法中使用的維生素決定,裁定,配備degasser ,第四紀泵,自動噴油系統( 0-100 UL )的,以二極管陣列和熒光檢測器串聯耦合,以及溫度控制艙室的分析柱。該檢測系統可以同時檢測各種波長的光線。整個系統是由惠普化學工作站軟件,該軟件還負責管理的數據收集和處理系統。

    評價參數用於驗證的方法測定維生素B1和B2的線性,檢測和量化的限制和可重複性。所有的分析進行了抽樣使用凍乾字母a.雙孢蘑菇。

    線性確定的建設校準曲線與標準的解決方案含有維生素在大眾範圍從1.1至210納克和從2.3到202納克的維生素B1和B2分別。三注射了每一級。氧化反應的維生素B1 ,以thiochrome是同一用於樣品分析,並進行了中所述科2.5 。

  • 1 decade ago

    硫胺(B1)和ribflavin (B2)標準從斯格碼化學製品Co.,美國獲取了。

    標準維生素解答在0.1 N HCl準備了。

    酵素takadiastase從Fluka,瑞士獲取了。

    半新的有機,液相色譜的流動相色譜分析的成績和分析成績的其他試劑。

    水在Milli-Q (微孔)系統purifed使用準備流動相。

    所有流動相是通過與0.45 um的毛孔大小的膜ltered的?。

    Edwards式樣超級Modullyo冷凍烘乾機被使用冰凍乾燥用于方法學的檢驗的樣品。

    HP在系列和分析專欄的溫度控制隔間塑造1100液體色譜分析儀用于維生素決心的高性能,決心,裝備排氣機,四個一組的泵浦,自動注射系統(0-100 uL),當一台二極管陣列和?

    uorescence探測器被結合。

    檢測系統考慮到同時偵查在各種各樣的波長。

    整個系統是由馬力Chemstation軟體控制的,也執行蒐集數據和治療系統。

    被用于的評估引數確認維生素B1和B2的決的方法學是線性、偵查和quanti?正離子極限和反覆性。

    所有分析被執行了使用bisporus被冰凍乾燥的A.樣品。

    修建定標曲線取決於線性用包含維生素的標準解法在許多範圍從1.1到210 ng和從維生素的B1各自2.3到202 ng和B2。 三射入被做了在每個水準。

    硫胺的氧化作用反應對硫色素的是在樣品使用的相同的分析和被執行了正如第2.5部分所描述。

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    希望對你有幫助

    Source(s): 腦袋
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