Jiminy Cricket, voiced by Cliff Edwards. Jiminy is a cricket who acts as Pinocchio's "conscience" and the partial narrator of the story.
Pinocchio, voiced by Dickie Jones. Pinocchio is a wooden puppet made by Geppetto and turned into a living puppet by the Blue Fairy.
Geppetto, voiced by Christian Rub. Geppetto is a toymaker who creates Pinocchio and wishes for him to become a real boy.
Figaro and Cleo, voiced by Mel Blanc. Geppetto's black and white housecat and goldfish, respectively.
J. Worthington "Honest John" Foulfellow, voiced by Walter Catlett. Honest John is a sly anthropomorphic fox who tricks Pinocchio twice in the film. His full name is possibly John Worthington Foulfellow.
Gideon is Honest John's dumb, mute and crafty anthropomorphic feline accomplice. His voice was originally to be supplied by Mel Blanc of Loony Tunes fame, but they deleted his dialogue in favor of a mute performance (i.e. Harpo Marx) just like Dopey of Snow White and the Seven Dwarfs. However, Gideon's hiccups were provided by Blanc.
Stromboli, voiced by Charles Judels. Stromboli is a large, sinister, bearded puppet maker who forces Pinocchio to perform onstage in order to make money. He speaks in an Italian Accent.
The Blue Fairy, voiced by Evelyn Venable. She is the beautiful fairy who brings Pinocchio to life and turns him into a real boy at the end.
The Coachman, voiced by Charles Judels. A corrupt coachman who owns and operates Pleasure Island. He speaks in a Cockney accent.
Lampwick, voiced by Frankie Darro. Lampwick is a naughty boy Pinocchio meets on his way to Pleasure Island. He turns into a donkey while the boys are hanging out.
is the whale that swallows Geppetto, Figaro, and Cleo during their search for Pinocchio.