Fluorescence in situ hybridization analysis.
For the detection of the various MALT lymphoma-associated translocations,the following probes flanking the breakpoint regions were used: bacterial artificial chromosomes RP11-1077C10,RP11-36L4,RP11-1080I 1,and RP11-40K4 for BCL10,RP11-215K24 and RP11-522N9 for FOXP1,RP11-465K1 and RP11-335D10 for CNN3,RP11-79K3 and RP11-77E14 for JMJD2C,RP11-13H2 0 and RP11-14K9 for ODZ2,and commercially available probes for IGH, MALT1,and BCL6 (Vysis). The cutoff value for the diagnosis of each probe set was the mean percentage of cells with a false-positive signal constellation plus 3 SDs,as assessed
on tissue from 20 reactive lymph nodes. Fluorescence in situ hybridization (FISH) procedure was done according to published standard methods
以上是原文 我不太明白其中rp11ㄉ意義 再來是forㄉ用法他放那邊我不知怎麼翻 總之麻煩高人指點一下!!請勿用軟體翻....因為不通!
- hillngreenLv 61 decade agoFavorite Answer
RP可能是bacterial artificial chromosomes的統一編號
08/11/2008 DECIPHER: Definition for International clonesets (NCBI36)
mucosa-associated lymphoid tissue (MALT)
MALT1 and BCL10 aberrations in MALT lymphomas and their effect on the expression of BCL10 in the tumour cells
Cases with the latter FISH pattern were further investigated using IGH probes (SO-labelled RP11-11771 and SG-labelled RP11-312H5, kindly provided by T Poulsen, University of Copenhagen, Denmark)21 and API2 (SO-labelled RP11-400E19/RP11-640G3 and SG-labelled RP11-315O6/RP11-10O13) (www.ensembl.org). The status of the BCL10 gene was analysed with a set of BAC clones flanking the gene (SO-labelled RP11-1080I/1RP11-40K4 and SG-labelled RP11-1077C10/RP11-36L4).
Interphase fluorescence in situ hybridization
Using bioinformatic resources available at the University of California at Santa Cruz (http://genome.ucsc.edu), BAC clones RP11-1080I1 and RP11-40K4 (telomeric) as well as RP11-1077C10 and RP11-36L4 (centromeric) flanking the BCL10 locus were selected as interphase FISH probes. The clones were differentially labeled with Spectrum Orange (SO, telomeric) and Spectrum Green (SG, centromeric) and pooled to obtain a break-apart assay. Bacteria culture, BAC DNA isolation and labeling, and probe preparation were performed as previously described.28 The diagnostic reliability of the newly developed BCL10 break-apart assay was recently proven in a series of controls including 4 cases of cytogenetically proven t(1;14)(p22;q32)5 (R.S. et al, unpublished data, March 2003). For detection of alterations affecting the MALT1 locus, the commercially available LSI-MALT1 probe was applied (Vysis, Downers Grove, IL).
- Anonymous5 years ago
而且滿1000 免運費 多種運送方式，
- Anonymous6 years ago