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嚕嚕米 asked in 科學生物學 · 1 decade ago

傾注法倒皿法(pour plate)

問題一:為何NA之溫度藥控制在45-50℃財能注入含樣品的培養皿中?

問題二:為何用此法時,在平板的表面及裏面皆可能有細菌生長?

◎是否有善心人士能在星期三之前為我解答呢?拜託 了!感激不盡阿!

1 Answer

Rating
  • VBC
    Lv 7
    1 decade ago
    Favorite Answer

    解答一:因為pour plate方式是將稀釋的菌液與融化的瓊脂培養基混合後倒入培養皿。

    溫度過高,則微生物死亡或生長受抑制;溫度太低,則瓊脂過早凝固無法均勻分佈。

    解答二:因為是將菌液先與瓊脂混合,所以內外都可能會有細菌生長。

    2008-10-29 09:54:59 補充:

    修正:

    解答一,菌液是先點於培養皿中心處,非與培養基混合

    2008-10-29 09:56:35 補充:

    倒入培養基後因為旋轉混合,所以內外都會有菌生長

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