mini asked in 社會與文化語言 · 1 decade ago

有請聰明大大幫幫忙!!~~~ 翻譯一下

Materials and methods

Mice and reagents

Sixty-two male, 6-week-old, C3H/HeJ mice were pursached from Charles River laboratories (Barcelona, Spain), and maintained under a 12-h light-dark cycle with free access to water and standard laboratory food. All mice were kept at the animal department of Experimental Medicine Unit of Hospital Gregorio Marañón (Madrid, Spain), which followed the European Regulations for Animal Experimentation (Directive: 86/609/EEC). The experiments were approved by the animal care committee of our institution.

A. simplex crude extract, regularly used for human skin testing (I.P.I. Diagnostics, Madrid. Spain), was used for the sensitization, challenge and ELISA experiments. This extract was produced, after thorough washings with sterile water, by homogenization of stage 3 larva followed by sonication and

delipidation as elsewhere described [35]. The A. simplex extract was next biologically standardized and it is now in use in allergy clinics. It has been tested in fish allergic patients with negative responses. Pertussis toxin (PT) and Concanavalin A were pursached from Sigma-Aldrich Inc.

(Missouri, USA).

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  • 1 decade ago
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    給你永遠免煩人翻譯法:

    http://translate.google.com.tw/translate_t#

    應該不會差很多 好用給個點數吧 ㄎㄎ

    翻譯文如下:

    材料和方法

    小鼠和試劑

    62名男性, 6個週齡, C3H/HeJ小鼠pursached從查爾斯河實驗室(西班牙巴塞羅那) ,並保持在12小時光暗週期免費獲得水和食品的標準實驗室。所有小鼠保持在動物系實驗醫學股格雷戈里拉尼翁醫院(馬德里,西班牙) ,隨後歐洲條例動物實驗(指令: 86/609/EEC ) 。實驗所核准的照顧動物委員會的機構。

    字母a.單純粗提物,經常用於人體皮膚測試(新聞學會診斷,馬德里。西班牙) ,用於宣傳,挑戰和酶聯免疫吸附試驗。這種提取物生產,徹底沖洗後的無菌水,由同質化階段3幼蟲其次是超聲和與其他地方一樣delipidation描述[ 35 ] 。單純的字母a.提取未來生物標準化和現在使用的過敏症診所。據測試,魚類過敏患者的消極的反應。百日咳毒素( PT )和伴刀豆球蛋白A是pursached從Σ -奧爾德里奇公司

    (密蘇里州,美國) 。

  • 1 decade ago

    材料和方法老鼠和試劑六十二個男性, 6星期老, C3H/HeJ老鼠從查理斯河實驗室(巴塞羅那,西班牙) pursached,并且赡养了在與自由存取的一個12-h光黑暗的週期之下對水和標準實驗室食物。 所有老鼠收留在實驗性醫學單位的動物部門醫院格雷戈裡奧Marañón (馬德里,西班牙),遵守动物实验的(方針歐洲章程: 86/609/EEC)。 實驗由我們的機關动物护养委員會批准。 A.單缸粗暴萃取物,通常用于人的皮膚測試(I.P.I.診斷,馬德里。 西班牙),為促進感受性、挑戰和ELISA實驗使用了。 這種萃取物在详尽的洗滌物以後导致了,用不育的水,由階段3幼蟲的勻化聲處理跟随的和 delipidation如在別處被描述[35]。 A.單缸萃取物是下生物規範化,并且它現在是在使用中在過敏診所。 它在有否定响应的魚過敏病人被測試了。 百日咳毒素(PT)和伴刀豆球蛋白A從斯格碼Aldrich Inc. pursached。 (密蘇里,美國)。

    Source(s): 字典
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