志維 asked in 社會與文化語言 · 1 decade ago

可以幫我翻這些嗎? 感恩~~

Anthocyanins were extracted and purified according to

the method of Zhang et al. (2004) with some modifications.

A total of 100 g fresh litchi peel tissues was extracted overnight

in an extraction medium consisting of 0.5 M HCl at

25 度C. Extract was filtered through Whatman No. 1 paper,

and the filter residue was re-extracted until the absorbance

of the extract at 510 nm was 60.01. Filtrates were combined

and concentrated to a small volume at 40度C by a

rotary evaporator. The concentrated filtrates were then

loaded onto an Amberlite XAD-7 resin column

(1.5 · 40 cm) (Sigma), and washed with distilled water, followed

by an elution with 0.1% HCl in methanol. The fractions

with the highest absorbance at 510 nm were pooled

and evaporated to remove methanol and dried under vacuum

at 35 度C. The residue was re-dissolved in 20 ml of

methanol for the analysis of antioxidant properties.

The antioxidant activity of anthocyanins against lipid

peroxidation was measured through ammonium thiocyanate

assay, as described by Takao, Kitatani, Watanabe,

Yagi, and Sakata (1994) with some modifications. The

reaction solution, containing 0.2 ml of 100 lg/ml anthocyanin

extract, 0.2 ml of linoleic acid emulsion (25 mg/ml in

99% ethanol) and 0.4 ml of 50 mM phosphate buffer (pH

7.4), was incubated in the dark at 40 C. A 0.1 ml aliquot

of the reaction solution was then added to 3 ml of 70%

(v/v) ethanol and 0.1 ml of 30% (w/v) ammonium thiocyanate.

Precisely 3 min after the addition of 0.1 ml of 20 mM

ferrous chloride in 3.5% (v/v) hydrochloric acid to the reaction

mixture, the absorbance of the resulting red colour

was measured at 500 nm. Aliquots were assayed every

24 h until the day after the absorbance of the control solution

(without anthocyanin extract) reached maximum

value. Butylated hydroxytoluene (BHT) was used as positive

control.

1 Answer

Rating
  • 1 decade ago
    Favorite Answer

    大致是這樣吧 !

    花青素被提取了和被淨化了根據

    方法張・等(2004) 以一些修改。

    一共計100 個g 新鮮的荔枝果皮組織隔夜被提取了

    在提取媒介包括0.5 M HCl 在

    25?C 。萃取物被過濾通過Whatman 第1 紙,

    並且過濾器殘滓被反萃取了直到absorbance

    萃取物在510 毫微米是60.01 。濾出液被結合了

    並且集中對小容量在40?C 由a

    旋轉蒸發儀。被集中的濾出液然後是

    裝載Amberlite XAD-7 樹脂專欄

    (1.5 · 40 cm) (斯格碼), 和洗滌與蒸餾水, 被跟隨

    由一elution 與0.1% HCl 在甲醇裡。分數

    以最高的absorbance 在510 毫微米合併了

    並且蒸發取消甲醇和烘乾在真空之下

    在35?C 。殘滓被再溶解在20 機器語言

    甲醇為對抗氧化物產的分析。

    花青素的抗氧化活動反對油脂

    過氧化被測量了通過氨鹽基硫氰酸

    分析用試樣, 如所描述由Takao, Kitatani, 渡邊,

    Yagi, 和Sakata (1994) 以一些修改。

    反應解答, 包含0.2 機器語言100 lg/ml 花青素

    萃取物, 0.2 機器語言亞麻酸酸乳化液(25 mg/ml

    99% 對氨基苯甲酸二) 和0.4 機器語言50 毫米磷酸鹽緩衝(酸鹼度

    7.4), 被孵化了在黑暗在40 C 。一個0.1 機器語言整除數

    反應解答然後增加了來3 機器語言70%

    (v/v) 對氨基苯甲酸二和0.1 機器語言30% (w/v) 氨鹽基硫氰酸。

    精確地3 分鐘在0.1 機器語言的加法以後20 毫米

    亞鐵氯化物在3.5% 裡(v/v) 鹽酸對反應

    混合物, 收效的紅顏色的absorbance

    被測量了在500 毫微米。整除數是被檢驗的每

    24 h 直到天在控制解答的absorbance 以後

    (沒有花青素萃取物) 被到達的最大值

    價值。被丁基化的hydroxytoluene (BHT) 被使用了作為正面

    控制。

    • Commenter avatarLogin to reply the answers
Still have questions? Get your answers by asking now.