? asked in 科學生物學 · 1 decade ago



1. ureteric bud (UB)

2. mesenchymal-to-epithelial transformation

3. metanephric mesenchyme (MM)

4. E-cadherin immunostaining






1. morphogen-like

2. promotes

3. epithelialization

4. crosslink

5. maturation

6. cryopreserved

7. branching

資料來源 ~~ Biomaterials 28 (2007) 4806–4817

Update 2:



Hyaluronic acid (HA) is a glycosaminoglycan of tissue engineering importance that plays a vital role in mammalian development.

Update 3:

We found that HA has the ability to simultaneously modulate ureteric bud (UB) branching, promote mesenchymal-to-epithelial transformation, and promote differentiation of both metanephric mesenchyme (MM) and the UB depending on the concentration and molecular weight (MW) of HA.

Update 4:

Hyaluronidase inhibited branching morphogenesis in both isolated UB and whole kidney cultures, suggesting endogenous HA is required for branching morphogenesis.

Update 5:

HA exhibited morphogen-like properties, stimulating branching morphogenesis at low concentrations (0.1%) and low MW (6.55 kDa),

Update 6:

E-cadherin immunostaining and qPCR of nephron differentiation markers (OAT-1, NaPi-2, AQP-1, and THP) demonstrated that HA of a variety of MWs strongly promotes mesenchymal epithelialization and nephron differentiation in a concentration-dependent manner.

Update 7:

Since the HA synthesis and degradation genes, has-2 and hyal-2, are highly expressed during kidney development, this data suggests that specific sizes and concentrations of HA

Update 8:

may act to independently regulate UB branching and promote tubular maturation, representing a potential switch for ending branching morphogenesis, as well as initiating nephron differentiation.

Update 9:

In addition, the ability of HA to promote in vitro embryonic kidney growth and maturation, together with the biocompatibility and crosslinking capability of HA,

Update 10:

suggests a potential use of HA for both creating an instructive, 3D scaffold for in vitro kidney engineering from developmental tissues, as well as promoting tubule regeneration in injured or cryopreserved kidneys.

1 Answer

  • Sigma
    Lv 7
    1 decade ago
    Favorite Answer

    1. ureteric bud (UB): 可以腎芽稱之, 是腎臟發育的早期結構.

    2. mesenchymal-to-epithelial transformation: 間質細胞和上皮之間的交互作用, 是腎芽長出成為永久腎臟所必經的作用

    3. metanephric mesenchyme (MM): 也就是永久腎臟發育過程中的間質細胞, 必需和上皮交互作用, 導致腎芽長出成為永久腎臟

    4. E-cadherin immunostaining : E-cadherin位於細胞表面, 是細胞與細胞之間的黏附因子(cell adhesion molecules), 中文可稱為鈣黏著素E, 因為其黏著功能和鈣有關 ; immnuostaining是免疫染色, 也就是說用針對E-cadherin 的特異性 抗體來染色證明其存在的位置, 如果細胞表面有E-cadherin 的話, 就會有顏色出來, 反之, 就沒有顏色, 也就是說染不出來, 表示這些細胞表面沒有E-cadherin 的分子

    Source(s): 100% 自己腦袋中的知識, 100% 自己打的文字
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