what is hemolytic test?

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  • Anonymous
    1 decade ago
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    The sugar-water hemolysis test is a blood test to detect fragile red blood cells by testing their ability to withstand swelling in a low-salt solution.

    How the Test is Performed

    Blood is drawn from a vein, usually on the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic. An elastic band is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the band to swell with blood.

    A needle is inserted into the vein, and the blood is collected in an airtight vial or a syringe. During the procedure, the band is removed to restore normal blood flow. Once the blood has been collected, the needle is removed. The puncture site is covered to stop any bleeding.

    For an infant or young child, the area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a small glass tube (pipette), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if bleeding continues.

    The blood is tested in a laboratory.

  • 1 decade ago

    A screen for haemolysis generally involves performing a full blood examination including a reticulocyte count (which would be raised) and an examination of a blood film under a microscope (would see polychromasia and spherocytes and if oxidative haemolysis is taking place, bite or blister cells). A direct antiglobulin test (sometimes called a direct Coomb's test) would be performed to see if the cause of the haemolysis is antibodies attached to the red cells. Some other biochemical tests should be performed like a haptoglobin level (which binds free haemaglobin and so is decreased in the presence of haemolysis.

    The sucrose lysis test is a specific test for the diagnosis of PNH (paroxysmal nocturnal haemaglobinuria). PNH cells are very sensitive to complement. All red cells will adsorb complement components from serum at low ionic concentrations. PNH cells, because of their great sensitivity will undergo lysis whereas normal red cells will not.

    Source(s): Medical Scientist (Haematology).
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