糖果妹 asked in 科學化學 · 1 decade ago

教育學習論文

中文摘要(需要中翻英..專業ㄉ唷)

本研究使用咸豐草以不同烘乾溫度(40、60、80和100℃)處理後,再以50%乙醇為溶劑進行熱回流萃取,探討烘乾溫度對抗氧化活性之影響;咸豐草及楓香以?同溶劑(正己烷、二氯甲烷、甲醇-水及甲醇-氯仿)依序進?咸豐草熱回?萃取,探討?同溶劑萃取物之抗氧化活性。抗氧化成分含量測試包括總多酚化合物及類黃酮化合物含量分析,抗氧化活性測試包括清除DPPH自由基能?、螯合亞鐵離子能力、相對還原?、清除超氧陰?子能?、抑制微脂?氧化作用及清除ABTS陽?子等,並與BHA、EDTA、α-tocopherol及沒食子酸之抗氧化性做比較。

結果顯示,以不同溫度處理咸豐草莖部與葉部所得之樣品,再經50%乙醇進行熱回流萃取,所得萃取物之萃取率,葉高於莖,於各試驗皆以40℃烘乾之葉部表現活性最佳,其類黃酮及總多酚含量為10.89 mg/g與63.96 mg/g,在清除DPPH自由基試驗中,其IC50為0.03 ± 0.00 mg/g,在螯合亞鐵離子能力試驗中,其IC50為0.08 ± 0.01 mg/g,在相對還原能力試驗中,k值為1.13 ± 0.01 mL/mg,在清除超氧陰離子能力試驗,其清除率為60.70%,在抑制微脂?氧化作用試驗,其抑制率為70.99%,在清除ABTS陽?子試驗,其IC50為0.03 ± 0.01 mg/g。另外,以不同溶劑依序萃取咸豐草與楓香,萃取物之總多酚含量皆以甲醇-水片段萃取物含量最高,分別為63.60與49.41 mg/g,類黃酮含量以甲醇-氯仿片段萃取物含量最高,分別為49.91與26.81 mg/g,於抗氧化活性試驗中,皆以甲醇-水片段萃取物之表現活性最佳,在清除DPPH自由基試驗中,其IC50分別為0.03 ± 0.00 mg/g與0.06 ± 0.00 mg/g,在螯合亞鐵離子能力試驗中,其IC50分別為0.06 ± 0.01 mg/g與0.23 ± 0.00 mg/g,在相對還原能力試驗中,k值分別為0.88 ± 0.05與0.40 ± 0.01 mL/mg,在清除超氧陰離子能力試驗,其清除率分別為64.14與72.58%,在抑制微脂?氧化作用試驗,其抑制率分別為57.75%與69.67%,在清除ABTS陽?子試驗,其IC50分別為0.05 ± 0.01與0.02 ± 0.00 mg/g。

綜合研究結果顯示,咸豐草與楓香萃取物皆具有抗氧化性,又以咸豐草經40℃烘乾處理過所得之葉部萃取物所表現之活性最高,不同溶劑萃取則以甲醇-水片段萃取物所表現之活性最高,且具有高含量的總多酚含量,由此結果顯示,抗氧化活性與總多酚含量間具有相關聯性。此結果有助於了解咸豐草與楓香之抗氧化性及選擇適合之加工方式。

關鍵字:咸豐草、楓香、抗氧化、總多酚、類黃酮

1 Answer

Rating
  • 阿ken
    Lv 4
    1 decade ago
    Favorite Answer

    This study used a different Xianfeng grass drying temperature (40,60,80 and 100 ℃) treatment, and then to 50 percent ethanol as solvent extraction return to heat, drying temperature of antioxidant activity of the affected grass and maple Xianfeng - To «the same solvent (n-hexane, methylene chloride, methanol - water and methanol - chloroform) were Progressive» Xianfeng heat back to grass »extraction, of« with the solvent extraction of the antioxidant activity. Antioxidant content of tests including the composition of polyphenols and flavonoids, compounds content analysis, including the antioxidant activity test can remove DPPH radical », chelated iron ion capacity, the relative reduction», removing superoxide Yam »son can», Inhibit micro-fat »oxidation and removal of ABTS-» son, and BHA, EDTA, α-tocopherol and the gallic acid oxidation resistance compared.

    The results showed that in different temperatures and the Department of Xianfeng stem from the leaf samples, and 50 percent ethanol as a hot back extraction, extracts from the extraction rate, higher than the leaf stem, in the pilot 40 ℃ drying The leaves of the best performance, its total flavonoids and polyphenol content of 10.89 mg / g and 63.96 mg / g, in the removal of DPPH radical experiment, the IC50 was 0.03 ± 0.00 mg / g, in sequestration Asia Iron capacity test, the IC50 was 0.08 ± 0.01 mg / g, in the relative reduction in the ability test, k value of 1.13 ± 0.01 mL / mg, in the removal of superoxide ability test, the clearance rate was 60.70 percent, in suppressing Micro-fat »oxidation test, its inhibition rate of 70.99 percent, in the removal of ABTS-«-pilot,

    2008-05-14 23:11:06 補充:

    the IC50 was 0.03 ± 0.01 mg / g. In addition, in a different solvent extraction Xianfeng grass and then Liquidambar formosana, extracts of the total polyphenol content methanol - fragments of water extract of the highest content,

    2008-05-14 23:11:18 補充:

    respectively 63.60 and 49.41 mg / g, flavonoid content of methanol -- Chloroform fragment extract the highest content, respectively 49.91 and 26.81 mg / g, in the antioxidant activity of test,

    2008-05-14 23:11:47 補充:

    methanol - water extract of a fragment of the best performance in the removal of DPPH radical experiment, their respective IC50 To 0.03 ± 0.00 mg / g and 0.06 ± 0.00 mg / g, ferrous ions in the sequestration ability test,

    2008-05-14 23:12:00 補充:

    their IC50 were 0.06 ± 0.01 mg / g and 0.23 ± 0.00 mg / g, in the relative reduction in the ability test, k values were 0.88 ± 0.05 and 0.40 ± 0.01 mL / mg, in the removal of superoxide ability test,

    2008-05-14 23:12:11 補充:

    their clearance rates were 64.14 and 72.58 percent, in inhibiting micro-fat »oxidation test, the inhibition rates were 57.75 percent and 69.67 percent, the clearance ABTS-«-pilot, the IC50 were 0.05 ± 0.01 and 0.02 ± 0.00 mg / g.

    2008-05-14 23:12:24 補充:

    Study results showed that Xianfeng grass with Liquidambar extract all of anti-oxidation, on the 40 ℃ Xianfeng grass drying handled through the leaf extract of the performance of the highest activity,

    2008-05-14 23:12:35 補充:

    while different solvent extraction methanol - water Fragment extracts shown by the highest activity, and has a high content of the total polyphenol content, which showed that the total antioxidant activity and polyphenol content of inter-linked with.

    2008-05-14 23:12:46 補充:

    The results will help understand the Liquidambar Xianfeng grass with anti-oxidation and select the appropriate method of processing.

    Source(s): 自己
Still have questions? Get your answers by asking now.