英翻中 vol.3 (生化) 20點

Antimicrobial susceptibility testing was performed by a modification of the method proposed by the Hancock Laboratory (MIC Determination for Cationic Antimicrobial Peptides by Modified Micro Titer Broth Dilution Method).21 Briefly, the bacterial strains were grown inMueller-Hinton broth (HIMEDIA) until exponential growth was achieved.

利用Hancock 實驗室 (MIC Determination for Cationic Antimicrobial Peptides by Modified Micro Titer Broth Dilution Method)提出的modification of the method完成了Antimicrobial susceptibility testing。簡而言之,the bacterial strains會在Mueller-Hinton broth (HIMEDIA)下生長直到達到指數生長.

Test compound was dissolved in water/DMSO to make a series of twofold dilution. Ninty microliters of 2–7 · 105 CFUs/ml of bacterial sample per ml of Mueller-Hinton broth (HIMEDIA) was dispensed into 96-well polypropylene microtiter plate (SIGMA). Then 10 ll of serially diluted peptides in 0.01% acetic acid and 0.2% bovine serum albumin of desired concentration were added.

試驗複合物溶於水/DMSO造成一連串的兩倍稀釋. 90 microliters of 2–7 · 105 CFUs/ml of bacterial sample per ml of Mueller-Hinton broth (HIMEDIA) 被分配成96-well polypropylene microtiter plate (SIGMA). 然後增加 10 ll of serially diluted peptides in 0.01% acetic acid and 0.2% bovine serum albumin of desired concentration。

The microtiter plates were incubated overnight at 37 _C and the absorbance was read at 630 nm. Cultures without the peptides were used as positive control. Uninoculated Mueller-Hinton broth was used as negative control. The tests were carried out in triplicate. The minimum inhibitory concentration (MIC) is taken as the lowest concentration of compound that inhibits 80% growth of microorganism.

Update:

很感謝大家的幫忙!!

翻譯軟體翻的可能還需要再修飾過!!(詞不達意)

希望還能看到更理想的翻譯!!

謝謝!!

3 Answers

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  • 1 decade ago
    Best Answer

    藥敏試驗是由一個方法的改進所提出的漢考克實驗室(麥克風測定陽離子抗菌肽的改性微滴度肉湯稀釋法) 0.21簡單來說,菌株的生長inmueller -寒春肉湯( himedia ) ,直到指數增長實現了。利用漢考克實驗室(麥克風測定陽離子抗菌肽的改性微滴度肉湯稀釋法)提出的方法的改進完成了藥敏試驗。簡而言之,菌株會在穆勒-寒春肉湯( himedia )下生長直到達到指數生長。

    測試化合物溶解於水/二甲基亞砜作出了一系列的雙重稀釋。 90 microliters的2-7 105 cfus /毫升的細菌樣本,每毫升的穆勒-寒春肉湯( himedia )配發到96 -以及聚丙烯微板(西格瑪) 。然後10月11串行稀釋肽在0.01 % ,乙酸和0.2 % ,牛血清白蛋白理想的濃度補充說。

    試驗複合物溶於水/二甲基亞砜造成一連串的兩倍稀釋。 90 microliters的2-7 105 cfus /毫升的細菌樣本,每毫升的穆勒-寒春肉湯( himedia )被分配成96 -以及聚丙烯微板(西格瑪) 。然後增加10個當地僱員串行稀釋肽在0.01 % ,乙酸和0.2 % ,牛血清白蛋白理想的濃度。

    該微板的孵育,一夜之間在37 _c和吸收宣讀了630納米。沒有文化的多肽被用作陽性對照。 uninoculated穆勒-寒春肉湯被用來作為陰性對照。測試進行式三份。最低抑菌濃度( MIC )是採取作為的最低濃度複合抑制80 %微生物的生長。

    Source(s): 翻譯的
  • 1 decade ago

    答案一、二都是翻譯軟體

  • 妤蓁
    Lv 5
    1 decade ago

    抗菌靈敏性 測試一以漢考克實驗室(擴音器決心給Cationic 抗菌縮氨酸以修改的微Titer 肉湯稀釋方法)提議的方法的修改執行 . 21短暫,細菌勞累增長inMueller欣頓肉湯(HIMEDIA) 直到指數的發展被取得。

    利用漢考克實驗室 (擴音器決心給Cationic 抗菌縮氨酸以修改的微Titer 肉湯稀釋方法) 提出的這種方法的修改嗎?完成了測試的抗菌靈敏性嗎?簡而言之 ,細菌的勞累會在米勒-欣頓肉湯(HIMEDIA)下生長直到達到指數生長.

    測試化合物被在water/DMSO 溶解使一系列雙重稀釋。 Ninty microliters 2- 7 米勒-欣頓肉湯(HIMEDIA)的每ml細菌的樣品的105 CFUs/ml 被分發到96 健康的聚丙烯microtiter盤子(總和) . 然後10 ll的順序稀釋縮氨酸在0.01%的乙酸內和0.2%的如牛血清清蛋白的要求的集中被增加。

    試驗複合物溶於水/DMSO造成一連串的兩倍稀釋 . 90 microliters 2- 7 米勒-欣頓肉湯(HIMEDIA)的每ml細菌的樣品的105 CFUs/ml ? ? ? ? 96 健康的聚丙烯microtiter盤子(總和) . ? ? ? ? 在要求的集中的0.01%的乙酸和0.2%的如牛的血清清蛋白內的順序稀釋的縮氨酸的10 ll嗎?

    microtiter盤子孵化一夜對37 _ C和吸光度被以630納米讀。 沒有縮氨酸的文化被用作正控制。 Uninoculated米勒-欣頓肉湯被用作負的控制。 試驗被一式三份進行。 最小禁止的集中(擴音器) 被認為是抑制微生物的80%的增長的化合物的最低的濃度。

    Source(s): 翻譯軟體**
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