Biocompatibility. Extracts of the marker particles in 0.9%
saline solution were incubated with mouse fibroblasts and the
number of living cells was determined by their ability to
bromide (MTT test).13 As shown in Figure 5, cells cultured with
extracts of the marker particles did not exhibit reduced viability
as compared to the control. Thus, it can be concluded that the
particles do not release cytotoxic substances. In addition, extracts
of the particles in in inorganic (saline solution) or organic
(dimethylsulfoxide) solvents did not lead to an increased
mutation rate in a Salmonella typhimurium reverse mutation
assay (data not shown), which indicates that no mutagenic
substances are released from the particles.
Covalent immobilization of the fluorescent dye cresyl violet
to tosyl Dynabeads M-280 activated with tosyl chloride yielded
fluorescent magnetic microparticles that could be detected with
high sensitivity (detection limit in water and blood: 0.1 and 5
mg, respectively). The particles were developed as markers for
particle transfer to the patient to provide first fault safety in the
extracorporeal blood purification system MDS. They were
shown to be biocompatible and stable upon steam sterilization.
- 1 decade agoFavorite Answer
粹取出的標記粒子放在0.9%食鹽水裡, 與老鼠纖維母細胞一起培養. 存活的細胞數量以是否有能力代謝3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT測試). 圖5顯示與粹取出的標記粒子一起培養的細胞與對照組相比並無減低生存能力. 因此可以結論出: 此標記粒子並不會釋放細胞毒素. 另外, 粹取粒子在無機(食鹽水)與有機(二甲亞楓)溶劑裡都沒有在鼠傷寒沙門氏菌回復突變試驗裡導致突變率的增高 (數據未出示). 此結果表示這些粒子並不會釋出導致突變的因子.
甲酚紫螢光染劑用共價鍵固著在磺醯磁球用磺醯氯激活後, 產生了可高敏感度檢測出的螢光磁性微粒子 (在水和血裡的檢測限度分別為0.1和5豪克). 這些粒子被發展為病患粒子轉移的標記, 以提供體外血液淨化系統MDS的第一道安全門檻. 它們顯示擁有生物相容性, 並在蒸氣消毒後有穩定性.
2008-04-16 17:35:36 補充：