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Anonymous asked in 健康疾病與處置癌症 · 1 decade ago


Activation of Caspase-8 Contributes to

3,3#-Diindolylmethane-Induced Apoptosis

in Colon Cancer Cells.


3,3#-Diindolylmethane (DIM) is the major in vivo product of acid-catalyzed oligomerization of indole-3-carbinol, which is a

promising anticancer agent present in cruciferous vegetables and has itself been reported to have anticarcinogenic

properties. This study examined DIM-mediated regulation of apoptosis in the HCT116 (wild-type p53) and HT-29 (mutant

p53) human colon cancer cell lines. DIM (0–30 mmol/L) substantially decreased the number of viable cells and induced

apoptosis of HCT116 and HT-29 cells in a concentration-dependent manner. Western-blot analyses of total cell lysates

revealed that DIM increased the activation of caspase-3, -7, -8, and -9 and enhanced poly(ADP-ribose) polymerase

cleavage in both HCT116 and HT-29 cells. In addition, DIM increased the translocation of cytochrome c and Smac/Diablo

from the mitochondria to the cytoplasm. In concert with the caspase-8 activation by DIM, increased levels of Fas and

truncated Bid were observed. DIM did not affect the protein levels of p53, Bcl-2, Bax, or Fas ligand (FasL) in HCT116 cells.

In HT-29 cells, however, DIM decreased Bcl-2 levels, although the protein levels of Bax or FasL were not affected. The

caspase-8 inhibitor Z-IETD-FMK attenuated the DIM-induced apoptosis, indicating that increased activation of this enzyme

contributed to the increase in p53-independent apoptosis that was observed in colon cancer cells. We have demonstrated

that DIM induces apoptosis in colon cancer cells, providing insights into the mechanisms underlying its antitumorigenic

activities. J. Nutr. 137: 31–36, 2007.

4 Answers

  • Favorite Answer


    3,3# Diindolylmethane導致的Apoptosis



    3,3#-Diindolylmethane (昏暗)是吲哚的酸摧化的齊聚3 carbinol主要活體內產品,是a


    物產。 這項研究在HCT116 (狂放類型p53) 和HT-29 (突變體審查了apoptosis的昏暗斡旋的章程

    p53) 人的結腸癌細胞系。 昏暗(0 ¡ V30 mmol/L)極大地減少了可實行的細胞的數量并且導致


    顯露昏暗增加了caspase-3的活化作用, -7, -8和-9和提高了多(ADP核糖)聚合酶

    分裂在HCT116和HT-29細胞。 另外,黯淡增加細胞色素 c和Smac或者躲閃者的遷移

    從線粒體到細胞質。 協力caspase-8 活化作用由昏暗,增加的水平的啟遠地和

    被削的出價被觀察了。 昏暗沒有影響p53、Bcl-2、Bax 或者啟遠地ligand (FasL)的蛋白質水平在HCT116細胞。

    在HT-29細胞,然而,黯淡減少的Bcl-2水平,雖然Bax或 FasL的蛋白質水平不受影響。


    對在結腸癌細胞被觀察的p53獨立apoptosis的增量貢獻。 我們展示了


    活動。 J. Nutr。 137: 31 ¡ V36 2007年。

  • 1 decade ago



  • 1 decade ago

    yhahaha西方弄髒(Western blot)之後,因為髒亂所以要遷移(transfer)嗎?


  • 1 decade ago


    據我所知,(Western-blot analyses)是西方墨點法也就是所謂的免疫轉漬法分析試驗,



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