Sabrina asked in 科學生物學 · 1 decade ago

請生物高手幫我翻這段英文paper的意思!

請問一下這段英文中內容的意思..希望是詳細的中文翻譯...我已經看過一遍了...是有關於E.coli和T4 phage的吸附作用分析...

但總覺得自己翻的文意很奇怪...所以希望各位高手幫幫忙...感謝!!

千萬不要翻譯軟體翻的..

Adsorption assay of T4 phage to various E. coli

To investigate adsorption of T4 phage to various E. coli,

adsorption assay was carried out. Purified E. coli were cultivated

in LB medium overnight. The cells were inoculated

into 10 ml LB medium to adjust a final concentration at

108 CFU/ml and incubated at 37 ◦C. After 60 min incubation,

T4 phage were infected with a final concentration of

107 PFU/ml. The suspension were periodically taken and

centrifuged at 15,000×g, 4◦C for 1 min. The unadsorbed

phages in the supernatant were measured by the plaque

assay. The phage titer at time 0 was defined as 100%.

2 Answers

Rating
  • 1 decade ago
    Favorite Answer

    針對多種大腸桿菌作用的T4 噬菌體之吸附試驗

    為研究 T4 噬菌體對於多種大腸桿菌作用的吸附情形, 可利用吸附試驗完成之. 純化後的大腸桿菌於 LB 培養液進行隔夜培養後, 接種至 10 ml LB 培養液內, 並調整其最終濃度為 10^8 CFU/ml, 於 37 ◦C 進行培養. 培養 60 分鐘後, 以最終濃度為 10^8 CFU/ml 進行感染. 定時取出懸浮液於 4◦C 下, 以 15000 ×g 的轉速離心 1 分鐘. 上清液中未吸附的噬菌體可用溶菌斑試驗進行測量. 當噬菌體的 titer 測得為 0 時, 即表示吸附結果達 100%.

    Source(s): 自己
  • 1 decade ago

    T4 吸附分析用試樣噬菌對各種各樣的E. 桿菌

    調查T4 的吸附噬菌對各種各樣的E. 桿菌, 吸附分析用試樣被執行。被淨化的E. 桿菌隔夜培養了在磅媒介。細胞被接種入10 機器語言磅媒介調整最後的集中在108 CFU/ml 和被孵化了在37?C 。在60 以後極小的孵出, T4 噬菌被感染107 PFU/ml 的最後的集中。懸浮被採取了和階段性地被分離了在15,000□, 4?C 1 分鐘。unadsorbed 噬菌在上層清液由匾分析用試樣測量了。噬菌的滴定量在時間0 被定義了作為100% 。

    Source(s):
Still have questions? Get your answers by asking now.